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isotype control  (Bio-Rad)


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    Structured Review

    Bio-Rad isotype control
    Isotype Control, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 138 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd163/Mouse+anti+Pig+CD163/bio_rxiv__64898__2026__04__22__719209-213-17-19
    Average 94 stars, based on 138 article reviews
    isotype control - by Bioz Stars, 2026-09
    94/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Associations between endometrial macrophages and persistence of endometritis in postpartum dairy cows
    Article Snippet: Nonspecific binding was blocked with 5% bovine serum albumin (A7906; Sigma-Aldrich), for 1 h in a humid chamber at room temperature. .. Each slide was probed either with monoclonal antibody anti-CD68 (Total Mφ, 1:25; monoclonal mouse anti-bovine CD68, ED1, MA5-16654, Thermo Fisher Scientific), anti-CD86 (M1,1:50; monoclonal mouse anti-bovine CD86, IL-A190, MCA2437GA, BioRad) and anti-CD163 (M2, 1:200; monoclonal mouse anti-bovine CD163, EDHu1, MCA1853, BioRad) diluted in 5% bovine serum albumin, and incubated overnight at 4 °C in a humid chamber. .. After a 3 times PBS washing, each for 5 min, slides were incubated with the peroxidase conjugate polyclonal goat anti-mouse secondary antibody (1:100; A2554, Sigma-Aldrich) for 1 h in a humid chamber at room temperature.

    Article Title: Profiles of Specialized Pro-resolving Mediators (SPM) in dairy cows with healthy postpartum or endometritis.
    Article Snippet: .. Briefly, membranes were placed in blocking solution (5% defatted milk with 0.1% Tween 20) for 2 h at room temperature and incubated overnight at 4°C with primary antibodies anti-CD86 (1:2000, MCA2437GA, BioRad) and anti-CD163 (1:1000, MCA1853, BioRad) diluted in blocking solution. .. A mouse monoclonal antibody against β-actin (1:10000, AB20272, Abcam) was added to normalize protein load.

    Article Title: Associations between endometrial macrophages and persistence of endometritis in postpartum dairy cows
    Article Snippet: .. Sections were incubated overnight at 4°C with primary antibodies antiCD68 (1:50; monoclonal mouse anti-bovine CD68, ED1, MA5-16654, Thermo Fisher Scientific) and anti-CD163 (1:200, MCA1853, BioRad) diluted in PBS. .. Sections were then washed 3 times in PBS for 5 min and incubated at room temperature for 1 h, with the secondary antibody (1:100, A2554, Sigma-Aldrich).

    Next-Generation Sequencing:

    Article Title: Resistance training suppresses accumulation of senescent fibro-adipogenic progenitors and senescence-associated secretory phenotype in aging rat skeletal muscle
    Article Snippet: .. The primary antibodies anti-p21 (ab109199; 1:100)(Abcam), anti-CD68 (ab31630; 1:50)(Abcam), and anti-CD163 (MCA342R; 1:100) (Bio-Rad Laboratories, Hercules, CA, USA) were diluted in 5% NGS/PBS. .. After incubation with primary antibody, the sections were washed with PBS and incubated for 1 h at 25 °C with secondary antibodies (goat anti-mouse IgG [Invitrogen, Carlsbad, CA, USA] for BA-F8, p21 (BD), CD68 and CD163, goat anti-mouse IgM [Invitrogen] for 6H-1, and goat anti-rabbit IgG [Invitrogen] for PDGFR-α, p21 (Abcam) and laminin) or wheat germ agglutinin (WGA) with Alexa Fluor (Invitrogen).

    Article Title: Resistance training suppresses accumulation of senescent fibro-adipogenic progenitors and senescence-associated secretory phenotype in aging rat skeletal muscle.
    Article Snippet: .. The primary antibodies anti-p21 (ab109199; 1:100)(Abcam), anti-CD68 (ab31630; 1:50)(Abcam), and anti-CD163 (MCA342R; 1:100) (Bio-Rad Laboratories, Hercules, CA, USA) were diluted in 5% NGS/PBS. .. After incubation with primary antibody, the sections were washed with PBS and incubated for 1 h at 25 °C with secondary antibodies (goat antimouse IgG [Invitrogen, Carlsbad, CA, USA] for BA-F8, p21 (BD), CD68 and CD163, goat antimouse IgM [Invitrogen] for 6H-1, and goat antirabbit IgG [Invitrogen] for PDGFR-α, p21 (Abcam) and laminin) or wheat germ agglutinin (WGA) with Alexa Fluor (Invitrogen).

    Blocking Assay:

    Article Title: Profiles of Specialized Pro-resolving Mediators (SPM) in dairy cows with healthy postpartum or endometritis.
    Article Snippet: .. Briefly, membranes were placed in blocking solution (5% defatted milk with 0.1% Tween 20) for 2 h at room temperature and incubated overnight at 4°C with primary antibodies anti-CD86 (1:2000, MCA2437GA, BioRad) and anti-CD163 (1:1000, MCA1853, BioRad) diluted in blocking solution. .. A mouse monoclonal antibody against β-actin (1:10000, AB20272, Abcam) was added to normalize protein load.

    Immunohistochemistry:

    Article Title: Glucuronolactone Promotes Mucin Sulfation to Alleviate Deoxynivalenol‐Induced Intestinal Injury via Microbiota‐Dependent and ‐Independent AHR Activation
    Article Snippet: For MALII lectin staining, the experimental procedures were performed with biotinylated MALII (B‐1265‐1, Vector Laboratories, 1:200) and YSFluor 488‐conjugated streptavidin (35103ES60, Yeasen Biotechnology, 5 μg/mL) according to the methods described in a previous study [ ]. .. The primary antibodies for immunohistochemistry and immunofluorescence: anti‐Claudin‐1 (28674‐1‐AP, Proteintech, 1:500), anti‐CD163 (MCA2311A647, BIO‐RAD, 1:200), anti‐MUC2 (27675‐1‐AP, Proteintech, 1:250), anti‐ZO‐1 (21773‐1‐AP, Proteintech, 1:200), anti‐F4/80 (29414‐1‐AP, Proteintech, 1:250), anti‐AHR (AF6278, Affinity Biosciences, 1:200), anti‐CYP1A1 (13241‐1‐AP, Proteintech, 1:200), anti‐GAL3ST3 (24851‐1‐AP, Proteintech, 1:200). .. Secondary antibodies: Alexa Fluor 488 AffiniPure Goat Anti‐Rabbit IgG (111‐545‐003, Jackson ImmunoResearch) and Cy3 AffiniPure Goat Anti‐Rabbit IgG (111‐165‐003, Jackson ImmunoResearch).

    Immunofluorescence:

    Article Title: Glucuronolactone Promotes Mucin Sulfation to Alleviate Deoxynivalenol‐Induced Intestinal Injury via Microbiota‐Dependent and ‐Independent AHR Activation
    Article Snippet: For MALII lectin staining, the experimental procedures were performed with biotinylated MALII (B‐1265‐1, Vector Laboratories, 1:200) and YSFluor 488‐conjugated streptavidin (35103ES60, Yeasen Biotechnology, 5 μg/mL) according to the methods described in a previous study [ ]. .. The primary antibodies for immunohistochemistry and immunofluorescence: anti‐Claudin‐1 (28674‐1‐AP, Proteintech, 1:500), anti‐CD163 (MCA2311A647, BIO‐RAD, 1:200), anti‐MUC2 (27675‐1‐AP, Proteintech, 1:250), anti‐ZO‐1 (21773‐1‐AP, Proteintech, 1:200), anti‐F4/80 (29414‐1‐AP, Proteintech, 1:250), anti‐AHR (AF6278, Affinity Biosciences, 1:200), anti‐CYP1A1 (13241‐1‐AP, Proteintech, 1:200), anti‐GAL3ST3 (24851‐1‐AP, Proteintech, 1:200). .. Secondary antibodies: Alexa Fluor 488 AffiniPure Goat Anti‐Rabbit IgG (111‐545‐003, Jackson ImmunoResearch) and Cy3 AffiniPure Goat Anti‐Rabbit IgG (111‐165‐003, Jackson ImmunoResearch).

    other:

    Article Title: Spatial analysis identifies DC niches as predictors of pembrolizumab therapy in head and neck squamous cell cancer
    Article Snippet: Anti-CD163 , Bio-Rad , Cat# MCA1853; RRID: AB_2074540.



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    Distribution of <t>CD163</t> + M2 TAMs in AKR-derived allograft tumor tissues from immunocompetent C57BL/6 mice. (a, b) Representative IHC staining images showing CD163 + M2 TAMs in the (a) peritumoral stroma and (b) tumor islets. Lower panels display higher-magnification views of the regions outlined by red dashed boxes. (c, d) Quantification of CD163 + cells in the (c) peritumoral stroma and (d) tumor islets. (e) Comparison of CD163 + cell density between the peritumoral stroma and tumor islets. (f) Total number of CD163 + cells in allograft tumors (peritumoral stroma and tumor islets combined). (g, h) Comparison of the density between iNOS + cells and CD163 + cells in the (g) peritumoral stroma and (h) tumor islets. (i, j) Quantification of iNOS + /CD163 + ratio in the (i) peritumoral stroma and (j) tumor islets. p < 0.05 (∗), p < 0.01 (∗∗), p < 0.001 (∗∗∗). A field of view is ∼0.086 mm 2 in (c−j).
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    Distribution of <t>CD163</t> + M2 TAMs in AKR-derived allograft tumor tissues from immunocompetent C57BL/6 mice. (a, b) Representative IHC staining images showing CD163 + M2 TAMs in the (a) peritumoral stroma and (b) tumor islets. Lower panels display higher-magnification views of the regions outlined by red dashed boxes. (c, d) Quantification of CD163 + cells in the (c) peritumoral stroma and (d) tumor islets. (e) Comparison of CD163 + cell density between the peritumoral stroma and tumor islets. (f) Total number of CD163 + cells in allograft tumors (peritumoral stroma and tumor islets combined). (g, h) Comparison of the density between iNOS + cells and CD163 + cells in the (g) peritumoral stroma and (h) tumor islets. (i, j) Quantification of iNOS + /CD163 + ratio in the (i) peritumoral stroma and (j) tumor islets. p < 0.05 (∗), p < 0.01 (∗∗), p < 0.001 (∗∗∗). A field of view is ∼0.086 mm 2 in (c−j).
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    (A) UMAP of CD45 + immune cells from HIS mouse tumors, split per CMS subtype (n = 14,578 cells). (B) Boxplots showing proportions of tumor-infiltrating immune cells across major lineages (T, NK, myeloid, and B cells) stratified by CMS subtype. (C) Representative immunohistochemical staining of FFPE sections from subcutaneous HIS CRC tumors of CMS1 and CMS4 subtypes using CD4, CD8, and <t>CD163,</t> with corresponding boxplot quantification of CD4 + , CD8 + , and <t>CD163</t> + cells expressed as cells/mm . Statistical significance was determined using a two-tailed Mann–Whitney test. Only statistically significant differences are shown (**p ≤ 0.01; ***p ≤ 0.001). (D) Dotplot of genes associated with human macrophage characterization across myeloid subsets in HIS tumors. (E) Dotplot of genes associated with human T cell exhaustion across T cell subsets in HIS tumors. Dot size indicates the proportion of cells expressing the gene, and color indicates expression level. B: B cell; T: T cell; Tn: T naive; Tm: T memory; Treg: regulatory T; CD4Tfh: CD4 + follicular helper T cell; CD4Th17: CD4 + Th17 cell; MAIT: mucosal associated invariant T; NKT: natural killer T; dnT: double negative T; NK: natural killer cell; pDC: plasmacytoid dendritic cell; mono-macs: monocytes-macrophages; cDC1: conventional dendritic cell type 1; cDC2: conventional dendritic cell type 2; UMAP: Uniform Manifold Approximation and Projection; HIS: humanized immune system; CMS: Consensus Molecular Subtype.
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    (A) UMAP of CD45 + immune cells from HIS mouse tumors, split per CMS subtype (n = 14,578 cells). (B) Boxplots showing proportions of tumor-infiltrating immune cells across major lineages (T, NK, myeloid, and B cells) stratified by CMS subtype. (C) Representative immunohistochemical staining of FFPE sections from subcutaneous HIS CRC tumors of CMS1 and CMS4 subtypes using CD4, CD8, and <t>CD163,</t> with corresponding boxplot quantification of CD4 + , CD8 + , and <t>CD163</t> + cells expressed as cells/mm . Statistical significance was determined using a two-tailed Mann–Whitney test. Only statistically significant differences are shown (**p ≤ 0.01; ***p ≤ 0.001). (D) Dotplot of genes associated with human macrophage characterization across myeloid subsets in HIS tumors. (E) Dotplot of genes associated with human T cell exhaustion across T cell subsets in HIS tumors. Dot size indicates the proportion of cells expressing the gene, and color indicates expression level. B: B cell; T: T cell; Tn: T naive; Tm: T memory; Treg: regulatory T; CD4Tfh: CD4 + follicular helper T cell; CD4Th17: CD4 + Th17 cell; MAIT: mucosal associated invariant T; NKT: natural killer T; dnT: double negative T; NK: natural killer cell; pDC: plasmacytoid dendritic cell; mono-macs: monocytes-macrophages; cDC1: conventional dendritic cell type 1; cDC2: conventional dendritic cell type 2; UMAP: Uniform Manifold Approximation and Projection; HIS: humanized immune system; CMS: Consensus Molecular Subtype.
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    (A) UMAP of CD45 + immune cells from HIS mouse tumors, split per CMS subtype (n = 14,578 cells). (B) Boxplots showing proportions of tumor-infiltrating immune cells across major lineages (T, NK, myeloid, and B cells) stratified by CMS subtype. (C) Representative immunohistochemical staining of FFPE sections from subcutaneous HIS CRC tumors of CMS1 and CMS4 subtypes using CD4, CD8, and <t>CD163,</t> with corresponding boxplot quantification of CD4 + , CD8 + , and <t>CD163</t> + cells expressed as cells/mm . Statistical significance was determined using a two-tailed Mann–Whitney test. Only statistically significant differences are shown (**p ≤ 0.01; ***p ≤ 0.001). (D) Dotplot of genes associated with human macrophage characterization across myeloid subsets in HIS tumors. (E) Dotplot of genes associated with human T cell exhaustion across T cell subsets in HIS tumors. Dot size indicates the proportion of cells expressing the gene, and color indicates expression level. B: B cell; T: T cell; Tn: T naive; Tm: T memory; Treg: regulatory T; CD4Tfh: CD4 + follicular helper T cell; CD4Th17: CD4 + Th17 cell; MAIT: mucosal associated invariant T; NKT: natural killer T; dnT: double negative T; NK: natural killer cell; pDC: plasmacytoid dendritic cell; mono-macs: monocytes-macrophages; cDC1: conventional dendritic cell type 1; cDC2: conventional dendritic cell type 2; UMAP: Uniform Manifold Approximation and Projection; HIS: humanized immune system; CMS: Consensus Molecular Subtype.
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    Novocastra anti cd163
    (A) UMAP of CD45 + immune cells from HIS mouse tumors, split per CMS subtype (n = 14,578 cells). (B) Boxplots showing proportions of tumor-infiltrating immune cells across major lineages (T, NK, myeloid, and B cells) stratified by CMS subtype. (C) Representative immunohistochemical staining of FFPE sections from subcutaneous HIS CRC tumors of CMS1 and CMS4 subtypes using CD4, CD8, and <t>CD163,</t> with corresponding boxplot quantification of CD4 + , CD8 + , and <t>CD163</t> + cells expressed as cells/mm . Statistical significance was determined using a two-tailed Mann–Whitney test. Only statistically significant differences are shown (**p ≤ 0.01; ***p ≤ 0.001). (D) Dotplot of genes associated with human macrophage characterization across myeloid subsets in HIS tumors. (E) Dotplot of genes associated with human T cell exhaustion across T cell subsets in HIS tumors. Dot size indicates the proportion of cells expressing the gene, and color indicates expression level. B: B cell; T: T cell; Tn: T naive; Tm: T memory; Treg: regulatory T; CD4Tfh: CD4 + follicular helper T cell; CD4Th17: CD4 + Th17 cell; MAIT: mucosal associated invariant T; NKT: natural killer T; dnT: double negative T; NK: natural killer cell; pDC: plasmacytoid dendritic cell; mono-macs: monocytes-macrophages; cDC1: conventional dendritic cell type 1; cDC2: conventional dendritic cell type 2; UMAP: Uniform Manifold Approximation and Projection; HIS: humanized immune system; CMS: Consensus Molecular Subtype.
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    (A) UMAP of CD45 + immune cells from HIS mouse tumors, split per CMS subtype (n = 14,578 cells). (B) Boxplots showing proportions of tumor-infiltrating immune cells across major lineages (T, NK, myeloid, and B cells) stratified by CMS subtype. (C) Representative immunohistochemical staining of FFPE sections from subcutaneous HIS CRC tumors of CMS1 and CMS4 subtypes using CD4, CD8, and <t>CD163,</t> with corresponding boxplot quantification of CD4 + , CD8 + , and <t>CD163</t> + cells expressed as cells/mm . Statistical significance was determined using a two-tailed Mann–Whitney test. Only statistically significant differences are shown (**p ≤ 0.01; ***p ≤ 0.001). (D) Dotplot of genes associated with human macrophage characterization across myeloid subsets in HIS tumors. (E) Dotplot of genes associated with human T cell exhaustion across T cell subsets in HIS tumors. Dot size indicates the proportion of cells expressing the gene, and color indicates expression level. B: B cell; T: T cell; Tn: T naive; Tm: T memory; Treg: regulatory T; CD4Tfh: CD4 + follicular helper T cell; CD4Th17: CD4 + Th17 cell; MAIT: mucosal associated invariant T; NKT: natural killer T; dnT: double negative T; NK: natural killer cell; pDC: plasmacytoid dendritic cell; mono-macs: monocytes-macrophages; cDC1: conventional dendritic cell type 1; cDC2: conventional dendritic cell type 2; UMAP: Uniform Manifold Approximation and Projection; HIS: humanized immune system; CMS: Consensus Molecular Subtype.
    Isotype Control, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    (A) UMAP of CD45 + immune cells from HIS mouse tumors, split per CMS subtype (n = 14,578 cells). (B) Boxplots showing proportions of tumor-infiltrating immune cells across major lineages (T, NK, myeloid, and B cells) stratified by CMS subtype. (C) Representative immunohistochemical staining of FFPE sections from subcutaneous HIS CRC tumors of CMS1 and CMS4 subtypes using CD4, CD8, and <t>CD163,</t> with corresponding boxplot quantification of CD4 + , CD8 + , and <t>CD163</t> + cells expressed as cells/mm . Statistical significance was determined using a two-tailed Mann–Whitney test. Only statistically significant differences are shown (**p ≤ 0.01; ***p ≤ 0.001). (D) Dotplot of genes associated with human macrophage characterization across myeloid subsets in HIS tumors. (E) Dotplot of genes associated with human T cell exhaustion across T cell subsets in HIS tumors. Dot size indicates the proportion of cells expressing the gene, and color indicates expression level. B: B cell; T: T cell; Tn: T naive; Tm: T memory; Treg: regulatory T; CD4Tfh: CD4 + follicular helper T cell; CD4Th17: CD4 + Th17 cell; MAIT: mucosal associated invariant T; NKT: natural killer T; dnT: double negative T; NK: natural killer cell; pDC: plasmacytoid dendritic cell; mono-macs: monocytes-macrophages; cDC1: conventional dendritic cell type 1; cDC2: conventional dendritic cell type 2; UMAP: Uniform Manifold Approximation and Projection; HIS: humanized immune system; CMS: Consensus Molecular Subtype.
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    Image Search Results


    Distribution of CD163 + M2 TAMs in AKR-derived allograft tumor tissues from immunocompetent C57BL/6 mice. (a, b) Representative IHC staining images showing CD163 + M2 TAMs in the (a) peritumoral stroma and (b) tumor islets. Lower panels display higher-magnification views of the regions outlined by red dashed boxes. (c, d) Quantification of CD163 + cells in the (c) peritumoral stroma and (d) tumor islets. (e) Comparison of CD163 + cell density between the peritumoral stroma and tumor islets. (f) Total number of CD163 + cells in allograft tumors (peritumoral stroma and tumor islets combined). (g, h) Comparison of the density between iNOS + cells and CD163 + cells in the (g) peritumoral stroma and (h) tumor islets. (i, j) Quantification of iNOS + /CD163 + ratio in the (i) peritumoral stroma and (j) tumor islets. p < 0.05 (∗), p < 0.01 (∗∗), p < 0.001 (∗∗∗). A field of view is ∼0.086 mm 2 in (c−j).

    Journal: Bioactive Materials

    Article Title: Immunomodulatory effects of biodegradable Mg–Cu–Zn alloy in esophageal cancer

    doi: 10.1016/j.bioactmat.2026.02.046

    Figure Lengend Snippet: Distribution of CD163 + M2 TAMs in AKR-derived allograft tumor tissues from immunocompetent C57BL/6 mice. (a, b) Representative IHC staining images showing CD163 + M2 TAMs in the (a) peritumoral stroma and (b) tumor islets. Lower panels display higher-magnification views of the regions outlined by red dashed boxes. (c, d) Quantification of CD163 + cells in the (c) peritumoral stroma and (d) tumor islets. (e) Comparison of CD163 + cell density between the peritumoral stroma and tumor islets. (f) Total number of CD163 + cells in allograft tumors (peritumoral stroma and tumor islets combined). (g, h) Comparison of the density between iNOS + cells and CD163 + cells in the (g) peritumoral stroma and (h) tumor islets. (i, j) Quantification of iNOS + /CD163 + ratio in the (i) peritumoral stroma and (j) tumor islets. p < 0.05 (∗), p < 0.01 (∗∗), p < 0.001 (∗∗∗). A field of view is ∼0.086 mm 2 in (c−j).

    Article Snippet: Tissue sections were then incubated with primary antibodies against iNOS (22226-1-AP, ProteinTech, China), CD163 (A26411PM, Abclone, China), CD8 (SP16, Maixin, China), CD4 (SP35, Maixin, China) or Ki-67 (12202S, Cell Signaling Technology) for 12 h at 4 °C, followed by secondary antibodies (Beyotime Biotechnology, Nantong, China).

    Techniques: Derivative Assay, Immunohistochemistry, Comparison

    (A) UMAP of CD45 + immune cells from HIS mouse tumors, split per CMS subtype (n = 14,578 cells). (B) Boxplots showing proportions of tumor-infiltrating immune cells across major lineages (T, NK, myeloid, and B cells) stratified by CMS subtype. (C) Representative immunohistochemical staining of FFPE sections from subcutaneous HIS CRC tumors of CMS1 and CMS4 subtypes using CD4, CD8, and CD163, with corresponding boxplot quantification of CD4 + , CD8 + , and CD163 + cells expressed as cells/mm . Statistical significance was determined using a two-tailed Mann–Whitney test. Only statistically significant differences are shown (**p ≤ 0.01; ***p ≤ 0.001). (D) Dotplot of genes associated with human macrophage characterization across myeloid subsets in HIS tumors. (E) Dotplot of genes associated with human T cell exhaustion across T cell subsets in HIS tumors. Dot size indicates the proportion of cells expressing the gene, and color indicates expression level. B: B cell; T: T cell; Tn: T naive; Tm: T memory; Treg: regulatory T; CD4Tfh: CD4 + follicular helper T cell; CD4Th17: CD4 + Th17 cell; MAIT: mucosal associated invariant T; NKT: natural killer T; dnT: double negative T; NK: natural killer cell; pDC: plasmacytoid dendritic cell; mono-macs: monocytes-macrophages; cDC1: conventional dendritic cell type 1; cDC2: conventional dendritic cell type 2; UMAP: Uniform Manifold Approximation and Projection; HIS: humanized immune system; CMS: Consensus Molecular Subtype.

    Journal: bioRxiv

    Article Title: Single-cell characterization of tumor immune landscapes in colorectal cancer humanized mice

    doi: 10.64898/2026.06.01.729295

    Figure Lengend Snippet: (A) UMAP of CD45 + immune cells from HIS mouse tumors, split per CMS subtype (n = 14,578 cells). (B) Boxplots showing proportions of tumor-infiltrating immune cells across major lineages (T, NK, myeloid, and B cells) stratified by CMS subtype. (C) Representative immunohistochemical staining of FFPE sections from subcutaneous HIS CRC tumors of CMS1 and CMS4 subtypes using CD4, CD8, and CD163, with corresponding boxplot quantification of CD4 + , CD8 + , and CD163 + cells expressed as cells/mm . Statistical significance was determined using a two-tailed Mann–Whitney test. Only statistically significant differences are shown (**p ≤ 0.01; ***p ≤ 0.001). (D) Dotplot of genes associated with human macrophage characterization across myeloid subsets in HIS tumors. (E) Dotplot of genes associated with human T cell exhaustion across T cell subsets in HIS tumors. Dot size indicates the proportion of cells expressing the gene, and color indicates expression level. B: B cell; T: T cell; Tn: T naive; Tm: T memory; Treg: regulatory T; CD4Tfh: CD4 + follicular helper T cell; CD4Th17: CD4 + Th17 cell; MAIT: mucosal associated invariant T; NKT: natural killer T; dnT: double negative T; NK: natural killer cell; pDC: plasmacytoid dendritic cell; mono-macs: monocytes-macrophages; cDC1: conventional dendritic cell type 1; cDC2: conventional dendritic cell type 2; UMAP: Uniform Manifold Approximation and Projection; HIS: humanized immune system; CMS: Consensus Molecular Subtype.

    Article Snippet: Primary antibodies CD4 (Abcam, 1:50), CD8 (DAKO, 1:50), CD20 (DAKO, 1:500), CD56 (Monosan, 1:50), CD163 (Cell Marque Corporation, 1:25) were diluted in antibody diluent (Agilent: CD4; Ventana: CD8, CD20, CD56, CD163) and incubated in a humidified chamber.

    Techniques: Immunohistochemical staining, Staining, Two Tailed Test, MANN-WHITNEY, Expressing